Regulatory

Part:BBa_K4752003:Design

Designed by: Lang Zeng, Yourong Yan, Zhe Jing   Group: iGEM23_HNU-China   (2023-10-06)


NCED3 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1378
    Illegal XbaI site found at 25
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1378
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1378
    Illegal BglII site found at 509
    Illegal BamHI site found at 1794
    Illegal XhoI site found at 178
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1378
    Illegal XbaI site found at 25
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1378
    Illegal XbaI site found at 25
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1641


Design Notes

This part comes from Arabidopsis. The gene NCED3(Gene ID: 820667) was initially identified, and about 2000bp upstream of the gene was selected to cover the promoter region,and we prepare this part through PCR. To enhance the system assembly, we added appropriate homologous arms and Saml and Pstl restriction endonuclease recognition sites.

Source

This part is naturally exists in Arabidopsis thaliana. After extracting the genome of Arabidopsis thaliana, we designed two sets of primers, targeting RUBY and Bx respectively,to amplify the NCED3 promoter sequence.

References